Your next bonus is visual. Researchers at the Vallabh/Minikel Lab recently discovered a method using CRISPRoff to target upstream prion genes. Based on the provided diagram of the CRISPRoff targeting and bisulfite sequencing results, answer the following three questions: 1) What noncleaving protein was used to target sequences? 2) What modification of H2B was most likely being studied? 3) Modifications to which nucleotide were detected by the protocol?